DNA Binding and Cleavage Studies of Cobalt Complexes Containing Bioactive Mixed Ligands

Authors

  • B. Sreekanth Sri Dharmasthala Manjunatheshwara College of Engineering and Technology, Karnataka, INDIA
  • G. Krishnamurthy Sahyadri Science College, Shimoga, Karnataka, INDIA
  • H. S. Bhojya Naik School of Chemical Sciences, Kuvempu University, Shankaraghatta, Shimoga, Karnataka, INDIA
  • T. K. Vishnuvardhan Acharya Institute of Technology, Bangalore, Karnataka, INDIA
  • M. R. Lokesh Acharya Institute of Technology, Bangalore, Karnataka, INDIA

Keywords:

Cobalt complexes, DNA binding, viscosity measurements, thermal denaturation and cleavage studies

Abstract

The complexes containing bioactive ligand of the type [Co(L1L2)] (PF6)2 complex (1) (where L1=1,10-phenanthroline and L2 = N,N-dimethyl-2-{5-[(pyrrolidin-1-ylsulfonyl)methyl]-1H-indol-3-yl}ethanamine (almotriptan)  in 1:1 molar ratio of ligands) and  [Co(L1L3L4)] (PF6)2 complex (2)  (where L3=4H-1,2,4-triazol-4-amine, L4=1H-benzimidazole-2-thiol and the ligands are in the ratio 1:1:1, were synthesized and characterized. The DNA binding property of the complexes with calf thymus DNA has been investigated using absorption spectra, viscosity measurements and thermal denaturation experiments. The intrinsic binding constant Kb has been estimated at room temperature. The absorption spectral studies indicate that the complexes intercalate between the base pairs of the CT-DNA tightly with intrinsic DNA binding constant of  2.4 × 106 M−1 for (1) and 3.3 × 104 M−1 for (2) in 5 mM Tris-HCl/50 mM NaCl buffer at pH 7.2, respectively. The oxidative cleavage activity of (1), and (2) upon pUC19 DNA were studied by using gel electrophoresis and the results show that complexes have potent nuclease activity.

Author Biographies

  • B. Sreekanth, Sri Dharmasthala Manjunatheshwara College of Engineering and Technology, Karnataka, INDIA
    Department of Chemical Engineering
  • G. Krishnamurthy, Sahyadri Science College, Shimoga, Karnataka, INDIA
    Department of Chemistry
  • H. S. Bhojya Naik, School of Chemical Sciences, Kuvempu University, Shankaraghatta, Shimoga, Karnataka, INDIA
    Department of PG Studies and Research in Industrial Chemistry
  • T. K. Vishnuvardhan, Acharya Institute of Technology, Bangalore, Karnataka, INDIA
    Department of Chemistry
  • M. R. Lokesh, Acharya Institute of Technology, Bangalore, Karnataka, INDIA
    Department of Chemistry

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Published

2019-06-06

Issue

Vol. 1 No. 1 (2014)

Section

Articles

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