Utilization of the Brewer’s Spent Grain for Lactic acid production: Acid hydrolysis and submerged fermentation
Keywords:
Lactic acid, Brewer’s Spent Grain, Lactobacillus plantarum ATCC 8014, Optimization.Abstract
The production of Lactic acid from Brewery Spent Grain using Lactobacillus plantarum ATCC 8014. was carried out in this study. Box Behnken Design (BBD) (Design expert® 7 software) was used to investigate the effect of temperature, reaction time and acid concentration during the hydrolysis. Fermentation of the hydrolyzate was performed at 35 °C, pH 5.0-5.5 and 200 rpm for 72 h.
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