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Optimization Study by Response Surface Methodology and Artificial Neural Network on the Culture Parameters of Citric Acid Bioproduction from Sweet Potato Peels

Uku, P. E., Dike, J. I

Abstract


In the cause of this research work, two steps enzymatic hydrolysis of sweet potato peel was carried out respectively. The effect of α-amylase dose, reaction time, and reaction temperature and biomass weight on glucose concentration was investigated carefully. The response surface methodology (REM) predicted the highest reducing sugar concentration at liquefaction to be 74 g/L, at the following optimized conditions; temperature of 45°C, α-amylase dose 0.7 %v/v, biomass weight 22.345 g and time of 15 minutes, while the laboratory experimental yield was 73.5 g/L which expressly after collection of results shows that the result is good and adoptable for this purpose. An electro thermal oven with the model DHG and temperature control and timer were used in a dry cell weight (DCW) analysis of the sweet potatoes peels to estimate the dry weight of the samples collected and analyzed. Eco tester pH2 was also used which was calibrated using buffer solutions. Weighing balance was also used during the experiment precisely Mettler Toledo model FA 2104 which was able to weigh up to 0.02 g to 210 g. Timing was also done by regulating the timing button and centrifuge was used to spin the sample in order to separate undissolved solution from solvent. A comparison graph was plotted to ascertain the differentials of citric and yeast against time and another comparison for citric and yeast against biomass. In all, the analysis proved that all the apparatus, methods, parameters, and investigatory measures adopted in this research work was useful and 98% reliable for citric acid bioproduction using sweet potato.


Keywords


Optimization techniques, production, chemical processing, methodology, artificial neural network, biomass, yeast formation, bioproduction techniques

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References


Anwar S, Ali S, Sardar AA. Citric acid fermentation of hydrolysed raw starch by Aspergillus niger IIB-A6 in stationary culture. Sindh Univ Res Jour. 2009;41(1):1–8.

Benuzzi DA, Segovia RF. Effect of the copper concentration on citric acid productivity by an Aspergillus niger strain. Appl Biochem Biotechnol. 1996;61(3):393–7. doi: 10.1007/BF02787810.

Betiku E, Adesina OA. Statistical approach to the optimization of citric acid production using filamentous fungus Aspergillus niger grown on sweet potato starch hydrolyzate. Biomass Bioenergy. 2013;55:350–4. doi: 10.1016/j.biombioe.2013.02.034.

Betiku E, Akindolani OO, Ismaila AR. Enzymatic hydrolysis optimization of sweet potato (Ipomoea batatas) peel using a statistical approach. Braz J Chem Eng. 2013;30(3):467–76. doi: 10.1590/S0104–66322013000300005.

Douglas Crabb WD, Mitchinson C. Enzymes involved in the processing of starch to sugars. Trends Biotechnol. 1997;15(9):349–52. doi: 10.1016/S0167–7799(97)01082–2.

Currie JN. The citric acid fermentation of Aspergillus niger. J Biol Chem. 1917;31(1):15–37. doi: 10.1016/S0021–9258(18)86708–4.

Das A, Roy P. Improved production of citric acid by a diploid strain of Aspergillus niger. Can J Microbiol. 1978;24(5):622–5. doi: 10.1139/m78–102.

Dasgupta J, Nasim S, Khan AW et al. Production of citric acid in molasses medium: effect of addition of lower alcohols during fermentation. J Microbiol Biotechnol. 1994;9:123–5.

Dawson MW, Maddox IS, Brooks JD. Effect of interruptions to the air supply on citric acid production by Aspergillus niger. Enzyme Microb Technol. 1986;8(1):37–40. doi: 10.1016/0141–0229(86)90008–6.

Georgieva M, Philipova K, Charakchieva S, et al. Influence of methanol on the phospholipid and fatty acid composition of Aspergillus niger IM-13 during citric acid fermentation. C r Acad Bulgare Sci. 1992;45:87–90.

Gerhartsz W. Enzymes in starch processing. In: Gerhartz W, editor, Enzymes in industry production and applications. Weinheim, Germany: VCH; 1990. p. 92–8.

Grewal HS, Kalra KL. Fungal production of citric acid. Biotechnol Adv. 1995;13(2):209–34. doi: 10.1016/0734–9750(95)00002–8.

Cahn FJ. Citric acid fermentation on solid materials. Ind Eng Chem. 1935;27(2):201–4. doi: 10.1021/ie50302a021.

John KS, Ali MN, Umakumar G, Tabassum H. Studies on inductive effect of methanol on production of citric acid from waste cellulosic substrates using locally isolated Aspergillus niger and MTTC Aspergillus niger strains. Int J Eng Sci Technol. 2012;4(2):431–41.

Kapoor KK, Chaudary K, Tauro P. Citric acid.in-industrial microbiology. 4th ed Reed G, editor. Westport, CT: AVI Publishing Co Inc; 1983. p. 709–47.


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