Extraction and Purification of Total Phenolic Content (TPC) from Seeds of Citrullus lanatus

Authors

  • Surya Prakash D.V Assistant Professor, Department of Biotechnology, Meerut Institute of Engineering and Technology, Meerut-250005, Uttar Pradesh
  • Amarjeet Redhu B.Tech Student, Department of Biotechnology, Meerut Institute of Engineering and Technology, Meerut-250005, Uttar Pradesh
  • Tanishka Chauhan B.Tech Student, Department of Biotechnology, Meerut Institute of Engineering and Technology, Meerut-250005, Uttar Pradesh
  • Anshu Mall B.Tech Student, Department of Biotechnology, Meerut Institute of Engineering and Technology, Meerut-250005, Uttar Pradesh
  • Aman Verma B.Tech Student, Department of Biotechnology, Meerut Institute of Engineering and Technology, Meerut-250005, Uttar Pradesh

Keywords:

Citrullus lanatus, Total Phenolic Content, Soxhlet Extraction, Column chromatography, Methanol.

Abstract

Citrullus lanatus is commonly known as Watermelon and belongs to Cucurbitaceae family. The phytochemicals are present in every part of the plant. Fruit is edible part of the plant. Watermelon fruit consists of flesh (68%), seeds (2%) and rind approximately 30% of the total mass of the fruit Phenolic compound content levels are very high and acts as anti-oxidants. the seeds also contain phytochemical elements such as alkaloids, flavonoids, tannins, and amino acids, vitamin C, lycopene, β-carotene, terpenoids, cardiac glycosides steroid hormones, carotenoids, lipids, oils and five kind of amino acid. This plant shows various pharmacological activites like anti-cancer ac, anti-inflammatory, anti-oxidant, anti-microbial and anti-diabetic activities etc. In this research, Total phenolic content was enhanced from seeds of Citrullus lanatus. In the batch extraction process, the optimitization of physico-chemical parameters are involved for extraction of TPC. Different Solvents, percentage solvents, time, pH and particle mesh sizes are associated to the extraction process of TPC. Here, the optimum parameters are methanol, 100% solvent, 36 hrs, pH-5 and 72 mesh size. The TPC concentration was found to be 28µg/ml in this batch extraction. Similarly, the TPC concentration was increased in the soxhlet extraction process and its concentration was 34µg/ml. Finally, the concentration of TPC was enhanced from the column chromatography and its concentration was 36µg/ml.

References

Braide, W., I. J. Odiong, S. U. Oranusi. Phytochemical and Antibacterial properties of the seed of watermelon (Citrullus lanatus). Prime Journal of Microbiology Research. 2012; 2(3): 99-104.

Alwan A, Maclean DR, Riley LM, et.al. Monitoring and surveillance of chronic non-communicable diseases: Progress and capacity in high burden countries. The Lancet. 2010; 376(9755): 1861-1868.

Alok Bhardwaj, Rajeev Kumar, Vivek Dabas, et.al. Evaluation of Anti-Ulcer Activity of Citrullus Lanatus Seed Extract in Wistar Albino Rats. International Journal of Pharmacy and Pharmaceutical Sciences. 2012; 4(5): 135-139.

Shon MY, Kim TH, Sung NJ. Antioxidants and free radical scavenging activity of Phellinus baumii (Phellinus of Hymenochaetaceae) extracts. Food Chemistry. 2003; 82: 593-597.

Miniati E. Assessment of phenolic compounds in Biological samples. Ann 1st Super Sanita. 2007; 43(4): 362-368.

Britton G, Khachik F. Carotenoids Vol 5: nutrition and health. Birkhauser. Basel-Boston-Berlin: Springer; 2009.

Olamide AA, Olayemi OO, Demetrius OO, et.al. Effects of methanolic extract of Citrullus lanatus seed on experimentally induced prostatic hyperplasia. European Journal of Medicinal Plants. 2011; 1(4):171-179.

Martınez-Valverde I, Periago MJ, Provan G, et.al. Phenolic compounds, lycopene and antioxidant activity in commercial varieties of tomato (Lycopersicon esculentum). Journal of the Science of Food and Agriculture. 2002; 82:323-330.

Kimura M, Rodriguez-Amaya DB. Harvest plus handbook for carotenoids analysis. Harvest plus-IFPRI, Washington DC; 2004.

Adesanya, A. Olamide, Olaseinde O. Olayemi, et.al. Effects of Methanolic Extract of Citrullus lanatus Seed on Experimentally Induced Prostatic Hyperplasia. European Journal of Medicinal Plants. 2011;1(4):171-179p.

Alok, BRK., Vivek, D., Niyaz, A. Evaluation of anti-ulcer activity of Citrallus lanatus seed extract in Wistar rats. Journal of Pharmacy and Pharmaceutical Sciences. 2012; 4.

Yadav, Tomar AK, Jithesh O, et.al. Purification and Partial Characterization of Low Molecular Weight Vicilin-Like Glycoprotein from the Seeds of Citrullus lanatus. The protein journal. 2011; 30(8):575-580p

Catherine, Rice-Evans, Nicholas J. Miller, et.al. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radical Biology and Medicine. 1996; 20(7): 933–956p.

Wolf Bors, Manfred Saran. Radical Scavenging by Flavonoid Antioxidants. Free Radical Research, 1987; 2(4-6): 289-294p.

Kumar, D. Kumar, O. Prakash. Evaluation of antioxidant potential, phenolic and flavonoid content of Hibiscus tiliaceus flowers. EJEAF Chem., 2008;7: 2863-2871p.

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Published

2021-10-20

Issue

Vol. 8 No. 2 (2021)

Section

Articles

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