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Importance of Engineering of the Protein Molecules: A Review

Vitthalrao Bhimasha Khyade

Abstract


Engineering of the proteins is a powerful tool for production of the novel proteins that serve as catalysts to induce selective chemical and biological transformations that would not otherwise be possible. The methods of rational and random (methods entailing evolution, selection and the screening) are employed for the purpose to alter the specificity of the substarte profiles of selectivity through mutating the enzymes. The proteins identified through these techniques are serving the most significant role in the applications (industrial and medicinal applications). Such proteins are also important for the study of relationship between protein structure and protein functions. The scientific field is expecting more modifications of natural enzymes and the proteins through protein engineering. The efficiencies and easyness in the protein modifications depend exclusively on the well-known methods of rational design and directed evolution (as well as new techniques). New technologies such as computational design, catalytic antibodies and mRNA display would be crucial for de novo engineering of enzymes and also for new areas of protein engineering. The attempt is concerned with a critical review on the methods for functional proteins and some practical applications of the protein engineering.


Keywords


PCR; Rational Protein Design; Saturation Mutagenesis; Squid Ring Teeth (SRT).

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References


Ulmer, KM. (1983). Protein engineering. Science, Vol. 219, No. 4585, (February 1983), pp.666-671, ISSN: 0036-8075

Gupta, MN. (1992).Enzyme function in organic-solvents. European Journal of Biochemistry, Vol. 203, No. 1-2, (January 1992), pp.25-32, ISSN: 0014-2956

Arnold, FH. (1993), Engineering proteins for non-natural environments. The FASEB Journal, Vol. 7, No. 9, (June 1993), pp.744-749, ISSN: 0892-6638

Antikainen, NM. & Martin, SF. (2005). Altering protein specificity: techniques and applications. Bioorganic & Medicinal Chemistry, Vol. 13, No. 8, (April 2005), pp.2701- 2716, ISSN: 0968-0896

Venkatesan, N. & Kim, BH. (2002). Synthesis and enzyme inhibitory activities of novel peptide isosteres. Current Medicinal Chemistry, Vol. 9, No. 24, (December 2002), pp.2243-2270, ISSN: 0929-8673

Shiba, K. (2004). MolCraft: a hierarchical approach to the synthesis of artificial proteins. Journal of Molecular Catalysis B: Enzymatic, Vol. 28, No. 4-6, (June 2004), pp.145-153, ISSN: 1381-1177

Mattanovich, D. & Borth, N. (2006). Applications of cell sorting in biotechnology. Microbial Cell Factories, Vol. 5, No.12, (March 2006), ISSN: 1475-2859

Wong, TS., Zhurina, D. & Schwaneberg, U. (2006). The diversity challenge in directed protein evolution. Combinatorial Chemistry & High Throughput Screening, Vol. 9, No. 4, (May 2006), pp.271-288, ISSN: 1386-2073

Shimizu, Y., Kuruma, Y., Ying, BW., Umekage, S. & Ueda, T. (2006). Cell-free translation systems for protein engineering. FEBS Journal, Vol. 273, No. 18, (September 2006), pp.4133-4140, ISSN: 1742-464X

Jackson, SE., D Craggs, T. & Huang, JR. (2006). Understanding the folding of GFP using biophysical techniques. Expert Review of Proteomics, Vol. 3, No. 5, (October 2006), pp.545-559, ISSN: 1478-9450

Yoshikuni, Y. & Keasling, JD. (2007). Pathway engineering by designed divergent evolution. Current Opinion in Chemical Biology, Vol. 11, No. 2, (April 2007), pp.233-239, ISSN: 1367-5931

Chockalingam, K., Blenner, M. & Banta, S. (2007). Design and application of stimulusresponsive peptide systems. Protein Engineering Design & Selection, Vol. 20, No. 4, (April 2007), pp.155-161, ISSN: 1741-0126

Laitinen, O. H., Nordlund, HR., Hytonen, VP. & Kulomaa, MS. (2007). Brave new (strept) avidins in biotechnology. Trends in Biotechnology, Vol. 25, No. 6, (June 2007), pp.269-277, ISSN: 0167-7799

Lushington, G. H., Gu, JX. & Wang, JL. (2007). Whither combine? New opportunities for receptor-based QSAR. Current Medicinal Chemistry, Vol. 14, No. 17, pp.1863-1877, ISSN: 0929-8673

Sidhu, SS. & Koide, S. (2007). Phage display for engineering and analysing protein interaction interfaces. Current Opinion in Structural Biology, Vol. 17, No. 4, (August 2007), pp.481-487, ISSN: 0959-440X

Gai, SA. & Wittrup, KD. (2007). Yeast surface display for protein engineering and characterization. Current Opinion in Structural Biology, Vol. 17, No. 4, (August 2007), pp.467-473, ISSN: 0959-440X

Chaput, JC., Woodbury, NW., Stearns, LA. & Williams, BAR. (2008). Creating protein biocatalysts as tools for future industrial applications. Expert Opinion on Biological Therapy, Vol. 8, No. 8, (August 2008), pp.1087-1098, ISSN: 1471-2598

Skerra, A. (2008). Alternative binding proteins: Anticalins-harnessing the structural plasticity of the lipocalin ligand pocket to engineer novel binding activities. FEBS Journal, Vol. 275, No. 11, (June 2008). pp.2677-2683, ISSN: 1742-464X

Goodey, NM. & Benkovic, SJ. (2008), Allosteric regulation and catalysis emerge via a common route. Nature Chemical Biology, Vol. 4, No. 8, (August 2008), pp.474-482, ISSN: 1552-4450

Li, H. B. (2008). ‘Mechanical engineering’ of elastomeric proteins: Toward designing new protein building blocks for biomaterials. Advanced Functional Materials, Vol. 18, No. 18, (September 2008), pp.2643-2657, ISSN: 1616-301X

Kazlauskas, RJ. (2005). Enhancing catalytic promiscuity for biocatalysis. Current Opinion in Chemical Biology, Vol. 9, No. 2, (April 2005), pp.195-201, ISSN: 1616-301X

Nobeli, I., Favia, A.D. & Thornton, JM. (2009). Protein promiscuity and its implications for biotechnology. Nature Biotechnology, 27, 2, (February 2009), pp.157-167, ISSN: 1087- 0156

Majors, B. S., Chiang, G. G. & Betenbaugh, M. J. (2009). Protein and genome evolution in mammalian cells for biotechnology applications. Molecular Biotechnology, Vol. 42, No. 2, (June 2009), pp.216-223, ISSN: 1073-6085

Carson, AE. & Barker, TH. (2009). Emerging concepts in engineering extracellular matrix variants for directing cell phenotype. Regenerative Medicine, Vol. 4, No. 4, (July 2009), pp.593-600, ISSN: 1746-0751

Tam, A. & Raines, RT. (2009). Protein engineering with the traceless Staudinger ligation. Methods in Enzymology: Non-natural amino acids, Vol. 462, pp.25-44, ISSN: 0076-6879

Van der Sloot, AM., Kiel, C., Serrano, L. & Stricher, F. (2009). Protein design in biological networks: from manipulating the input to modifying the output. Protein Engineering Design & Selection, Vol. 22, No. 9, (September 2009), pp.537-542, ISSN: 1741-0126

Labrou, NE. (2010). Random mutagenesis methods for in vitro directed enzyme evolution. Current Protein & Peptide Science, Vol. 11, No. 1, (February 2010), pp.91-100, ISSN 1389-2037

Golynskiy, MV. & Seelig, B. (2010). De novo enzymes: from computational design to mRNA display. Trends in Biotechnology, Vol. 28, No. 7, (July 2010), pp.340-345, ISSN: 0167- 7799

Wiseman, A. (1993). Designer enzyme and cell applications in industry and in environmental monitoring. Journal of Chemical Technology and Biotechnology, Vol.56, No.1, pp.3-13, ISSN: 0268-2575

James, J. & Simpson, BK. (1996). Application of enzymes in food processing. Critical Reviews in Food Science and Nutrition, Vol.36, No.5, pp.437-463, ISSN: 1040-8398.

Voigt, C. A., Kauffman, S. & Wang, Z. G. (2000). Rational evolutionary design: the theory of in vitro protein engineering evolution. Advanced Protein Chemistry, Vol.55, pp.79-160, ISSN: 0065-3233

Altamirano, MM., Blackburn, JM., Aguayo, C. & Fersht, AR. (2000). Directed evolution of a new catalytic activity using the α/β-barrel scaffold. Nature, Vol.403, (February 2000), pp.617-622, ISSN: 0028-0836

Kirk, O., Borchert, TV. & Fuglsang, CC. (2002). Industrial enzyme applications. Current Opinion in Biotechnology, Vol.13, No.4, (August 2002), pp.345-351, ISSN: 0958-1669

Tamas, L. & Shewry, PR. (2006). Heterologous expression and protein engineering of wheat gluten proteins. Journal of Cereal Science, Vol.43, No.3, (May 2006), pp.259-274, ISSN: 0733-5210

Olempska-Beer, Z. S., Merker, RI., Ditto, M. D. & DiNovi, M. J. (2006). Food-processing enzymes from recombinant microorganisms – a review. Regulatory Toxicology and Pharmacology, Vol.45, No.2, (July 2006), pp.144-158, ISSN: 0273-2300.

Rao, MB., Tanksale, AM., Ghatge, MS. & Deshpande, W. (1998). Molecular and biotechnological aspects of microbial proteases. Microbiology and Molecular Biology Reviews, Vol.62, No.3, (September 1998), pp.597-635, ISSN: 1092-2172

Rao, DECS., Rao, KV., Reddy, TP. & Reddy, VD. (2009). Molecular characterization, physicochemical properties, known and potential applications of phytases: an overview. Critical Reviews in Biotechnology, Vol.29, No.2, (June 2009), pp.182-198, ISSN: 0738-8551

Wintrode, PL., Miyazaki, K. & Arnold, FH. (2000). Cold adaptation of a mesophilic subtilisin-like protease by laboratory evolution. Journal of Biological Chemistry, Vol.275, No.41, (October 2000), pp.31635-31640, ISSN: 0021-9258

Ness, J. E., Welch, M., Giver, L., Bueno, M., Cherry, JR., Borchert, TV., Stemmer, WPC & Minshull, J. (1999). DNA shuffling of subgenomic sequences of subtilisin. Nature Biotechnology, Vol.17, pp.893-896, ISSN: 1087-0156

Gupta, R., Beg, QK. & Lorenz P. (2002). Bacterial alkaline proteases: molecular approaches and industrial applications. Applied Microbioology and Biotechnology, Vol.59, No.1, (June 2002), pp.15-32, ISSN: 0175-7598

Schallmey, M., Singh, A. & Ward, OP, (2004). Developments in the use of Bacillus species for industrial production. Canadian Journal of Microbiology, Vol.50, No.1, (January 2004), pp.1-17, ISSN: 0008-4166

Akoh, CC., Chang, SW., Lee, GC. & Shaw, JF. (2008). Biocatalysis for the production of industrial products and functional foods from rice and other agricultural produce. Journal of Agricultural and Food Chemistry, Vol.56, No.22, (November 2008), pp.10445-10451, ISSN: 0021-8561

Verma, M. L., Azmi, W. & Kanwar, SS. (2008). Microbial lipases: At the interface of aqueous and non-aqueous media – A review. Acta Microbiologica et Immunologica Hungarica, Vol.55, No.3, (September 2008), pp.265-294, ISSN: 1217-8950

Kurtovic, I., Marshall, SN., Zhao, X. & Simpson, BK. (2009). Lipases from mammals and fishes. Reviews in Fisheries Science, Vol.17, No.1, pp.18-40, ISSN: 1064-1262

Abdon Pena-Francesch and Melik C. Demirel (2019). Squid-Inspired Tandem Repeat Proteins: Functional Fibers and Films. Front. Chem., 21 February 2019. https://www.frontiersin.org/articles/10.3389/fchem.2019.00069/full

Timmis, KN., Steffan, RJ. & Unterman, R. (1994). Designing microorganisms for the treatment of toxic wastes. Annual Review of Microbiology, Vol.48, pp.525-557, ISSN: 0066-4227

Fetzner, S. (2000). Enzymes involved in the aerobic bacterial degradation of Nheteroaromatic compounds: Molybdenum hydroxylases and ring-opening 2,4- dioxygenases. Naturwissenschaften, Vol.87, No.2, (February 2000), pp.59-69, ISSN: 0028-1042

Cirino, PC. & Arnold, FH. (2002). Protein engineering of oxygenases for biocatalysis. Current Opinion in Chemical Biology, Vol.6, No.2, (April 2002), pp.130-135, ISSN: 1367-5931

Torres, E., Bustos-Jaimes, I. & Le Borgne, S. (2003). Potential use of oxidative enzymes for the detoxification of organic pollutants. Applied Catalysis B-Environmental, Vol.46, No.1, (October 2003), pp.1-15, ISSN: 0926-3373

Ahuja, SK., Ferreira, GM. & Moreira, AR. (2004). Utilization of enzymes for environmental applications. Critical Reviews in Biotechnology, Vol.24, No.2-3, pp.125-154, ISSN: 0738-8551

Le Borgne, S. & Quintero, R. (2003). Biotechnological processes for the refining of petroleum. Fuel Processing Technology, Vol.81, No.2, (May 2003), pp.155-169, ISSN: 0378-3820

Rehm, BHA. (2006). Genetics and biochemistry of polyhydroxyalkanoate granule selfassembly: the key role of polyester synthases. Biotechnology Letters, Vol.28, No.4, (February 2006), pp.207-213, ISSN: 0141-5492

Rehm, BHA. (2007). Biogenesis of microbial polyhydroxyalkanoate granules: a platform technology for the production of tailor-made bioparticles. Current Issues in Molecular Biology, Vol.9, pp.41-62, ISSN: 1467-3037.

Rehm, BHA. (2010). Bacterial polymers: biosynthesis, modifications and applications. Nature Reviews Microbiology, Vol.8, No.8, (August 2010), pp.578-592, ISSN: 1740-1526

Wu CH., Mulchandani A. & Chen W. (2008). Versatile microbial surface-display for environmental remediation and biofuels production. Trends in Microbiology, Vol. 16, No. 4, (April 2008), pp. 181-188, ISSN: 0966-842X

Ayala, M., Pickard, MA. & Vazquez-Duhalt, R. (2008). Fungal enzymes for environmental purposes, a molecular biology challenge. Journal of Molecular Microbiology and Biotechnology, Vol.15, No.2-3, pp.172-180, ISSN: 1464-1801

Lam, L., Liu, XY. & Cao, Y. (2003). Pretargeted radioimmunotherapy, a potential cancer treatment. Drugs of the Future, Vol.28, No.2, (February 2003), pp.167-173, ISSN: 0377-8282

Zafir-Lavie, I., Michaeli, Y. & Reiter, Y. (2007). Novel antibodies as anticancer agents. Oncogene, Vol.26, No.25, (May 2007), pp.3714-3733, ISSN: 0950-9232

Vazquez, E., Ferrer-Miralles, N., Mangues, R., Corchero, JL., Schwartz, S. & Villaverde, A. (2009). Modular protein engineering in emerging cancer therapies. Current Pharmaceutical Design, Vol.15, No.8, (March 2009), pp. 893-916, ISSN: 1381-6128

Buckel, P. (1996). Recombinant proteins for therapy. Trends in Pharmacological Sciences, Vol.17, No.12, (December 1996), pp.450-456, ISSN: 0165-6147

Filpula, D. & McGuire, J. (1999). Single-chain Fv designs for protein, cell and gene therapeutics. Expert Opinion on Therapeutic Patents, Vol.9, No.3, (March 1999), pp.231-245, ISSN: 1354-3776

Kurtzman, AL., Govindarajan, S., Vahle, K., Jones, JT., Heinrichs, V. & Patten, PA. (2001). Advances in directed protein evolution by recursive genetic recombination: applications to therapeutic proteins. Current Opinion in Biotechnology, Vol.12, No.4, (August 2001), pp.361-370, ISSN: 0958-1669

Bonin-Debs, AL., Boche, I., Gille, H. & Brinkmann, U. (2004). Development of secreted proteins as biotherapeutic agents. Expert Opinion on Biological Therapy, Vol.4, No.4, (April 2004, pp.551-558, ISSN: 1471-2598

Lowe, D. & Jermutus, L. (2004). Combinatorial protein biochemistry for therapeutics and proteomics. Current Pharmaceutical Biotechnology, Vol.5, No.1, (February 2004), pp.17-27, ISSN: 1389-2010

Paques, F. & Duchateau, P. (2007). Meganucleases and DNA double-strand break-induced recombination: Perspectives for gene therapy. Current Gene Therapy, Vol.7, No.1, (February 2007), pp.49-66, ISSN: 1566-5232

Futami, J., Kitazoe, M., Murata, H. & Yamada, H. (2007). Exploiting protein cationization techniques in future drug development. Expert Opinion on Drug Discovery, Vol.2, No.2, (February 2007), pp.261-269, ISSN: 1746-0441

Nuttall, SD. & Walsh, RB. (2008). Display scaffolds: protein engineering for novel therapeutics. Current Opinion in Pharmacology, Vol.8, No.5, (October 2008), pp.609- 615, ISSN: 1471-4892.

Liu, S., Maheshwari, R. & Kiick, KL. (2009). Polymer-based therapeutics. Macromolecules, Vol.42, No.1, (January 2009), pp.3-13, ISSN: 0024-9297

Sandhu, J. S. (1992). Protein engineering of antibodies. Critical Reviews in Biotechnology, Vol.12, No.5-6, pp.437-462, ISSN: 0738-8551

Zaccolo, M. & Malavasi, F. (1993). From cells to genes – how to make antibodies useful in human diagnosis and therapy. International Journal of Clinical & Laboratory Research, Vol.23, No.4, (November 1993), pp.192-198, ISSN: 0940-5437

Pini, A. & Bracci, L. (2000). Phage display of antibody fragments. Current Protein & Peptide Science, Vol.1, No.2, (September 2000), pp.155-169, ISSN: 1389-2037

Morea, V., Lesk, AM. & Tromantono, A. (2000). Antibody modelling: Implications for engineering and design. Methods, Vol.20, No.3, (March 2000), pp. 267-279, ISSN: 1046-2023

Olafsen, T. & Wu, AM. (2010). Antibody vectors for imaging. Seminars in Nuclear Medicine, Vol.40, No.3, (May 2010), pp.167-181, ISSN: 0001-2998

Vert, Michel; Doi, Yoshiharu; Hellwich, Karl-Heinz; Hess, Michael; Hodge, Philip; Kubisa, Przemyslaw; Rinaudo, Marguerite; Schué, François (2012). "Terminology for biorelated polymers and applications (IUPAC Recommendations 2012)" Pure and Applied Chemistry. 84 (2): 377–410. doi:10.1351/PAC-REC-10-12-04.

Chow, D., Nunalee, ML., Lim, DW., Simnick, AJ. & Chilkoti, A. (2008). Peptide-based biopolymers in biomedicine and biotechnology. Materials Science & Engineering Reports, Vol.62, No.4, (September 2008), pp.125-155, ISSN: 0927-796X

Banta, S., Megeed, Z., Casali, M., Rege, K. & Yarmush, ML. (2007). Engineering protein and peptide building blocks for nanotechnology. Journal of Nanoscience and Nanotechnology, Vol.7, No.2, (February 2007), pp.387-401, ISSN: 1533-4880

Banta, S., Wheeldon, IR. & Blenner, M. (2010). Protein engineering in the development of functional hydrogels. Annual Review of Biomedical Engineering, Vol.12, No.12, pp.167-186, ISSN: 1523-9829

Sarikaya, M., Tamerler, C., Jen, AKY., Schulten, K. & Baneyx, F. (2003). Molecular biomimetics: nanotechnology through biology. Nature Materials, Vol. 2, No.9, (September 2003), pp. 577-585, ISSN: 1476-1122

Seker, UOS., Wilson, B., Sahin, D., Tamerler, C. & Sarikaya, M. (2009). Quantitative affinity of genetically engineered repeating polypeptides to inorganic surfaces. Biomacromolecules, Vol.10, No.2, (February 2009), pp. 250-257, ISSN: 1525-7797

Oren, EE., Notman, R., Kim, IW., Evans, JS., Walsh, TR., Samudrala, R., Tamerler, C. & Sarikaya, M. (2010). Probing the molecular mechanisms of quartz-binding peptides. Langmuir, Vol.26, No.13, (July 2010), pp. 11003-11009, ISSN: 0743-7463.

Tamerler, C., Khatayevich, D., Gungormus, M., Kacar, T., Oren, EE., Hnilova, M. & Sarikaya, M. (2010). Molecular biomimetics: GEPI-based biological routes to technology. Biopolymers, Vol.94, No.1, (January 2010), pp. 78-94, ISSN: 0006-3525.

Hamada, D., Yanagihana, I. & Tsumoto, K. (2004). Engineering amyloidogenicity towards the development of nanofibrillar materials. Trends in Biotechnology, Vol.22, No.2, (February 2004), pp.93-97, ISSN: 0167-7799

Gilardi, G. & Fantuzzi, A. (2001). Manipulating redox systems: application to nanotechnology. Trends in Biotechnology, Vol.19, No.11, (November 2011), pp.468- 476, ISSN: 0167-7799

Wong, LL., Westlake, ACG. & Nickerson, DP. (1997). Protein engineering of cytochrome P450 (cam). Metal sites in proteins and models, Vol.88, pp.175-207, ISSN: 0081-5993

Wong, TS. & Schwaneberg, U. (2003). Protein engineering in bioelectrocatalysis. Current Opinion in Biotechnology, Vol.14, No.6, (December 2003), pp.590-596, ISSN: 0958- 1669

Saab-Rincon, G. & Valderrama, B. (2009). Protein engineering of redox-active enzymes. Antioxidants & Redox Signalling, Vol.11, No.2, (February 2009), pp.167-192, ISSN: 1523-0864

Gillam, EMJ. & Guengerich, EP. (2001). Exploiting the versatility of human cytochrome P450 enzymes: the promise of blue roses from biotechnology. IUBMB Life, Vol.52, No.6, (December 2001), pp.271-277, ISSN: 1521-6543.

Urlacher, VB. & Eiben, S. (2006). Cytochrome P450 monooxygenases: perspectives for synthetic application. Trends in Biotechnology, Vol.24, No.7, (July 2006), pp.324-330, ISSN: 0167-7799.

Kumar, S. (2010). Engineering cytochrome P450 biocatalysts for biotechnology, medicine and bioremediation. Expert Opinion on Drug Metabolism & Toxicity, Vol.6, No.2, (February 2010), pp.115-131, ISSN: 1742-5255

Martinkova, L. & Kren, V. (2010). Biotransformations with nitrilases. Current Opinion in Chemical Biology, Vol.14, No.2, (April 2010), pp.130-137, ISSN: 1367-5931.

Clapes, P.,Fessner, WD., Sprenger, GA. & Samland, AK. (2010). Recent progress in stereoselective synthesis with aldolases. Current Opinion in Chemical Biology, Vol.14, No.2, pp.154-167, ISSN: 1367-5931

Jordan, DB. & Wagschal, K. (2010). Properties and applications of microbial beta-Dxylosidases featuring the catalytically efficient enzyme from Selenomonas ruminantium. Applied Microbiology & Biotechnology, Vol.86, No.6, (May 2010), pp.1647-1658, ISSN: 0175-7598

Martinkova, L. & Kren, V. (2010). Biotransformations with nitrilases. Current Opinion in Chemical Biology, Vol.14, No.2, (April 2010), pp.130-137, ISSN: 1367-5931

Gupta, A. & Khare, SK. (2009). Enzymes from solvent-tolerant microbes: Useful biocatalysts for non-aqueous enzymology. Critical Reviews in Biotechnology, Vol.29, No.1, (March), pp.44-54, ISSN: 0738-8551

Doukyu, N. & Ogino, H. (2010). Organic solvent-tolerant enzymes. Biochemical Engineering Journal, Vol.48, No.3, (February 2010), pp.270-282, ISSN: 1369-703X

Pollegioni, L., Piubelli, L. & Molla, G. (2009). Cholesterol oxidase: biotechnological applications. FEBS Journal, Vol.276, No.23, (December 2009), pp.6857-6870, ISSN: 1742-464X

Leemhuis, H., Kelly, RM. & Dijkhuizen, L. (2010). Engineering of cyclodextrin glucanotransferases and the impact for biotechnological applications. Applied Microbiology and Biotechnology, Vol.85, No.4, (January 2010), pp.823-835, ISSN: 0175-7598

Masson, P., Carletti, E. & Nachon, F. (2009). Structure, activities and biomedical applications of human butyrylcholinesterase. Protein and Peptide Letters, Vol.16, No.10, pp. 1215- 1224, ISSN: 0929-8665

Kumar, P. & Satyanarayana, T. (2009). Microbial glucoamylases: characteristics and applications. Critical Reviews in Biotechnology, Vol.29, No.3, (September 2009), pp.225-255, ISSN: 0738-8551

Akoh, CC., Lee, GC. & Shaw, JF. (2004). Protein engineering and applications of Candida rugosa lipase isoforms. Lipids, Vol.39, No.6, (June 2004), pp.513-526, ISSN: 0024-4201

Song, JK., Han, JJ. & Rhee, JS. (2005). Phospholipases: Occurrence and production in microorganisms, assay for high-throughput screening, and gene discovery from natural and man-made diversity. Journal of the American Oil Chemists Society, Vol.82, No.10, (October 2005), pp.691-705, ISSN: 0003-021X

Bjarnason, JB., Asgeirsson, B. & Fox, JW. (1993). Psychrophilic proteinases from Atlantic cod. ACS Symposium Series, Vol.516, No.5, (December 1993), pp.69-82, ISSN: 0097-6156

Marcaida, MJ., Munoz, IG., Blanco, FJ., Prieto, J. & Montoya, G. (2010). Homing endonucleases: from basics to therapeutic applications. Cellular and Molecular Life Sciences, Vol.67, No.5, (March 2010), pp.727-748, ISSN: 1420-682X

Nygren PA. (2008). Alternative binding proteins: Affibody binding proteins developed from a small three-helix bundle scaffold. FEBS Journal, Vol.275, No.11, (June 2008), pp. 2668-2676, ISSN: 1742-464X

Gronwall, C. & Stahl, S. (2009). Engineered affinity proteins – Generation and applications. Journal of Biotechnology, Vol.140, No.3-4, (March 2009), pp. 254-269, ISSN: 0168-1656

Evans, TC., Xu, MQ. & Pradhan, S. (2005). Protein splicing elements and plants: From transgene containment to protein purification. Annual Review of Plant Biology, Vol.56, pp. 375-392, ISSN: 1040-2519

Perler FB. (2005). Protein splicing mechanisms and applications. IUBMB Life, Vol.57, No.7, (July 2005), pp.469-476, ISSN: 1521-6543

Klug, A. (2010). The discovery of zinc fingers and their applications in gene regulation and genome manipulation. Annual Review of Biochemistry, Vol.79, No.79, (July 2010), pp.213-231, ISSN: 0066-4154 Kumar, P. & Satyanarayana, T. (2009). Microbial glucoamylases: characteristics and applications. Critical Reviews in Biotechnology, Vol.29, No.3, (September 2009), pp.225-255, ISSN: 0738-8551

Craik, DJ., Cemazar, M. & Daly, NL. (2007). The chemistry and biology of cyclotides. Current Opinion in Drug Discovery & Development, Vol.10, No.2, (March 2007), pp.176-184, ISSN: 1367-6733


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