An Insight into the Production of Bioethanol from the Duckweed
Keywords:
Bioethanol, Duckweed, Hydrolysis, Fermentation, Saccharomyces CerevisiaeAbstract
The ultimate goal of this study was provided an insight into the production of bioethanol from the duckweed. In this study three experimental parameters for maximizing bioethanol yield were investigated during the dilute acid hydrolysis; acid concentration (0.6-1M), temperature (80-120oC) and time (60-180minutes). The conversion of duckweed to bioethanol can be achieved mainly by four process step; the first step was pretreatment of duckweed to remove different contaminates, dried at 60°C for 24hr and ground to the particle size of 2 mm. The pretreated duckweed was soaking in diluted acid hydrolysis to convert the starch into simple sugar. The simple sugars were fermented using Saccharomyces cerevisiae in anaerobic condition at 30°C, 4.5-5.3pH and 72hours, and the residual solutions were distilled every 150minutes by rotary evaporator. The concentrations of bioethanol were measured by Alcoholmeter and Pycnometer. Response Surface Methodology (RSM) using Design expert® 7.0 was used to optimize the bioethanol yield. The optimum result was obtained at 0.8M acid concentration, 100oC and 120minutes. The yield of bioethanol is 4.57ml/20gm (0.18gm/gm) was achieved under these conditions. The minimum result showed that bioethanol yield of 4.33/20gm sample (0.17gm/gm) was obtained at 0.91M, 103.59oC and 165 minutes respectively. Generally, the production of bioethanol from duckweed could be an option for energy and other uses.References
M. Veillette, M. Chamoumi, J. Nikiema, N. Faucheux, and M. Heitz, “Production of Biodiesel from Microalgae,” Adv. Chem. Eng., no. May 2014, 2012.
G. Gebrehiwot, S. A. Jabasingh, and A. Yimam, “Production of Cellulosic Ethanol from Wood Sawdust by Sulphuric acid Hydrolysis,” vol. 7, no. 3, pp. 30–35, 2017.
W. Cui, J. Xu, J. J. Cheng, and A. M. Stomp, “Growing duckweed for bioethanol production,” Am. Soc. Agric. Biol. Eng. Annu. Int. Meet. 2010, ASABE 2010, vol. 6, no. January 2010, pp. 4743–4749, 2010.
A. Kefale, M. Redi, and A. Asfaw, “Potential of bioethanol production and optimization test from agricultural waste: The case of wet coffee processing waste (pulp),” Int. J. Renew. Energy Res., vol. 2, no. 3, pp. 446–450, 2012.
A. Fissha and S. A. Jabasingh, “Production of Bioethanol from Barley Spent Grains ( BSG ) by two-stage dilute acid hydrolysis,” vol. 3, no. 1, 2018.
M. Kesaano, “Sustainable management of duckweed biomass grown for nutrient control in municipal wastewaters,” All Grad. Theses Diss. Pap. 879., 2011.
M. Vohra, J. Manwar, R. Manmode, S. Padgilwar, and S. Patil, “Bioethanol production: Feedstock and current technologies,” J. Environ. Chem. Eng., vol. 2, no. 1, pp. 573–584, 2014.
F. A. Hayilu and S. A. Jabasingh, “An Insight into the Production of Bioethanol from the Unused Ethiopian Waste Potatoes,” no. January 2018, 2019.
E. Sarikaya, T. Higasa, M. Adachi, and B. Mikami, “Comparison of degradation abilities of α- and β-amylases on raw starch granules,” Process Biochem., vol. 35, no. 7, pp. 711–715, 2000.
G. Gebrehiwot, K. Birhane, and T. Gebrekidan, Optimization of Sulphuric Acid Hydrolysis Process for Fermentable Sugars fromLignocellulosic Content of WoodSawdust for Production of Cellulosic Ethanol, Emerging Trends in Chemical Engineering vol. 7, no. 1, pp. 10–18, 2020.
M. Nasidi, “The potential of sorghum as a feedstock source for bioethanol production in Nigeria,” 2013.
E. Lam, K. J. Appenroth, T. Michael, K. Mori, and T. Fakhoorian, “Duckweed in bloom: The 2nd International Conference on Duckweed Research and Applications heralds the return of a plant model for plant biology,” Plant Mol. Biol., vol. 84, no. 6, pp. 737–742, 2014.
M. J. Taherzadeh and K. Karimi, Enzyme-based hydrolysis processes for ethanol from lignocellulosic materials: A review, vol. 2, no. 4. 2007.
J. J. Cheng and A. M. Stomp, “Growing Duckweed to recover nutrients from wastewaters and for production of fuel ethanol and animal feed,” Clean - Soil, Air, Water, vol. 37, no. 1, pp. 17–26, 2009.
Z. Bao et al., “Homology-integrated CRISPR-cas (HI-CRISPR) system for one-step multigene disruption in saccharomyces cerevisiae,” Syst. Biol. 2014 - Top. Conf. 2014 AIChE Annu. Meet., vol. 86, no. 11, pp. 3–12, 2014.
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