Chemical Composition Quantification of Components in Extracts of Uncaria tomentosa by HPLC and LC-MS Analysis

Authors

  • Swapnila Roy

DOI:

https://doi.org/10.37591/jocc.v10i1.7274

Abstract

The tropical medicinal vine known as cat's claw, Uncaria tomentosa (Willd. ex Schult.) DC. (Family: Rubiaceae), is native to the Amazon rainforest and other regions of South and Central America. It has been shown to be useful as an immune system booster, antioxidant, antibacterial, and anti-inflammatory agent and has been historically used to treat wounds, fever, urinary tract infections, abscesses, and asthma. U. tomentosa contains a variety of phytoconstituents, including proanthocyanidins, sterols, organic acids, oxindole and indole alkaloids, glycosides, and organic acids. Theileria, Plasmodium, and Babesia parasites, as well as pathogenic bacteria and viruses, have all been tested for their biological effects on U. tomentosa. Recently, a variety of cat's claw preparations—including tinctures, decoctions, capsules, extracts, and teas—have become marke table. This paper highlights solid phase extraction technique based on cationic and anionic mode which were used to quantify chemicalconstituents in extract, applying analytical technique using Liquid Chromatography-Mass Spectra(LC-MS) and high performance liquid chromatography (HPLC). There are reports of quantitative data as well as chromatogram resulted from methanolic extract of leaf, stem and root of plant which focused the alkaloids and other chemical constituents.

References

Amir M, Mujeeb M, Khan A, Ashraf K, Sharma D, Aqil M. Phytochemical analysis and in vitro antioxidant activity of Uncaria gambir. Int J Green Pharm 2012;6:67-72

Atanasov, A.G.; Waltenberger, B.; Pferschy-Wenzig, E.M.; Linder, T.; Wawrosch, C.; Uhrin, P.; Temml, V.; Wang, L.; Schwaiger, S.; Heiss, E.H.; et al. Discovery and resupply of pharmacologically active plant-derived natural products: A review. Biotech. Adv. 2015, 33, 1582–1614.

Navarro, V.J.; Barnhart, H.; Bonkovsky, H.L.; Davern, T.; Fontana, R.J.; Grant, L.; Reddy, K.R.; Seeff, L.B.; Serrano, J.; Sherker, A.H.; et al. Liver injury from herbals and dietary supplements in the U.S. Drug-Induced Liver Injury Network. Hepatology 2014, 60, 1399– 1408.

Azevedo, B.C.; Roxo, M.; Borges, M.C.; Peixoto, H.; Crevelin, E.J.; Bertoni, B.W.; Contini, S.H.T.; Lopes, A.A.; França, S.C.; Pereira, A.M.S.; et al. Antioxidant activity of an aqueous leaf extract from Uncaria tomentosa and its major alkaloids mitraphylline and isomitraphylline in Caenorhabditis elegans. Molecules 2019, 24, 3299.

Snow, A.D.; Castillo, G.M.; Nguyen, B.P.; Choi, P.Y.; Cummings, J.A.; Cam, J.; Hu, Q.; Lake, T.; Pan, W.; Kastin, A.J.; et al. The Amazon rain forest plant Uncaria tomentosa (cat’s claw) and its

specific proanthocyanidin constituents are potent inhibitors and reducers of both brain plaques and tangles. Sci. Rep. 2019, 9, 561.

Rojas-Duran, R.; González-Aspajo, G.; Ruiz-Martel, C.; Bourdy, G.; Doroteo-Ortega, V.H.; Alban- Castillo, J.; Robert, G.; Auberger, P.; Deharo, E. Anti-inflammatory activity of Mitraphylline isolated from Uncaria tomentosa bark. J. Ethnopharmacol. 2012, 143, 801– 804.

Batiha, G.E.S.; Beshbishy, A.A.; Tayebwa, D.S.; Shaheen, M.H.; Yokoyama, N.; Igarashi,

I. Inhibitory effects of Uncaria tomentosa bark, Myrtus communis roots, Origanum vulgare leaves and Cuminum cyminum seeds extracts against the growth of Babesia and Theileria in vitro. Jpn. J. Vet. Parasitol. 2018, 17, 1–13.

Moraes, R.C.; Dalla Lana, A.J.; Kaiser, S.; Carvalho, A.R.; de Oliveira, L.F.S.; Fuentefria, A.M.; Ortega, G.G. Antifungal activity of Uncaria tomentosa (Willd.) DC against resistant non-albicans Candida isolates. Ind. Crops Prod. 2015, 69, 7–14.

Farias, I.L.G.; Araújo, M.C.S.; Farias, J.G.; Rossato, L.V.; Elsenbach, L.I.; Dalmora, S.L.; Flores, N.M.; Durigon, M.; Cruz, I.B.; Morsch, V.M.; et al. Uncaria tomentosa for reducing side effects caused by chemotherapy in CRC Patients: Clinical trial. Evid. Based Complement. Alternat. Med. 2012, 2012, 892182.

Xiang, L.; Li, Y.; Deng, X.; Kosanovic, D.; Schermuly, R.T.; Li, X. Natural plant products in treatment of pulmonary arterial hypertension. Pulm. Circ. 2018, 8.

Navarro, M.; Arnaez, E.; Moreira, I.; Hurtado, A.; Monge, D.; Monagas, M. Polyphenolic composition and antioxidant activity of Uncaria tomentosa commercial bark products. Antioxidants 2019, 8, 339.

Zhang, Q.; Zhao, J.J.; Xu, J.; Feng, F.; Qu, W. Medicinal uses, phytochemistry and pharmacology of the genus Uncaria. J. Ethnopharmacol. 2015, 173, 48–80.

Downloads

Published

2023-07-29

Issue

Vol. 10 No. 1 (2023)

Section

Articles

License

Declaration and Copyright Transfer Form

(to be completed by authors)

I/ We, the undersigned author(s) of the submitted manuscript, hereby declare, that the above manuscript which is submitted for publication in the STM Journals(s), is not published already in part or whole (except in the form of abstract) in any journal or magazine for private or public circulation, and, is not under consideration of publication elsewhere.

  • I/We will not withdraw the manuscript after 1 week of submission as I have read the Author Guidelines and will adhere to the guidelines.
  • I/We Author(s ) have niether given nor will give this manuscript elsewhere for publishing after submitting in STM Journal(s).
  • I/ We have read the original version of the manuscript and am/ are responsible for the thought contents embodied in it. The work dealt in the manuscript is my/ our own, and my/ our individual contribution to this work is significant enough to qualify for authorship.
  •  I/We also agree to the authorship of the article in the following order:

Author’s name                                    

 

1. ________________

2. ________________

3. ________________

4. ________________

 

We  Author(s) tick this box and would request you to consider it as our signature as we agree to the terms of this Copyright Notice, which will apply to this submission if and when it is published by this journal.